[3DEM] A question about ice thickness of cryo-EM

[Hu, Guobin]

Dear Dr. Bill Tivol,

 

You are so right that the maximum ice thickness for imaging depends on
the HT of the scope. I sent a message to Paul asking about the specific
HT of 100 Kv. Hopefully He would tell me a number. Of course, I'd be
happier if our colleagues could tell me the numbers not only of 100 Kv,
but also of 200 Kv, 300 Kv and 400 Kv.

Thanks!

 

Regards,

Guobin

 

 

 

 

-----Original Message-----
From: owner-3dem at ncmir.ucsd.edu [mailto:owner-3dem at ncmir.ucsd.edu] On
Behalf Of Bill Tivol
Sent: Thursday, February 17, 2005 1:01 PM
To: 3dem at ucsd.edu
Subject: Re: [3DEM] A question about ice thickness of cryo-EM

 

 

On Feb 17, 2005, at 9:34 AM, Hu, Guobin wrote: 

	 

	Thanks for your information! You might have given me a basic
idea what the maximum of ice thickness could be.  Actually as indicated
in my earliest message, I'm not trying to figure out how thick the ice
could be in any specific case. What I'm trying to find out is an
estimate of the maximum of ice thickness of cryo-EM grids which is good
enough for imaging. I wonder if anybody has ever collected data with ice
thicker than 800 nm.

	 

	Thanks for any further help!

	 

Dear Guobin, 

The maximum ice thickness for imaging depends on the HT of your scope.
We have been imaging small whole cells at 300 kV, and we can do
tomography on cells that are a little smaller than 1 um. Of course, the
ice may not be that thick except in the immediate neighborhood of the
cell, and the resolution should improve for thinner ice, so the answer
to "Is the ice too thick for imaging?" is "It depends on what you're
trying to do and what equipment you have to do it." 

Yours, 

Bill Tivol, PhD 

EM Scientist and Manager 

Cryo-Electron Microscopy Facility 

Broad Center, Mail Code 114-96 

California Institute of Technology 

Pasadena CA 91125 

(626) 395-8833 

tivol at caltech.edu 

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