A question about ice thickness of cryo-EM

[Hu, Guobin]

Hi colleagues,

 

For our image analysis, we need to estimate the maximum ice thickness in
our cryo-EM specimen on 3.5/1 quantifoil grids. We may be able to
estimate that based on the thickness of carbon foil. As indicated by the
quantifoil product catalogue, the standard thickness of carbon foil is
12 nm. While observations of our cryo-EM micrographs have suggested that
the thickness of carbon foil is definitely much larger than 12 nm. Does
anybody have an idea of what the maximum thickness of ice could be on
quantifoil grids (3.5/1)? 

Thanks for help!

Guobin Hu

 

 

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