<html>
<head>
<meta http-equiv=Content-Type content="text/html; charset=us-ascii">
<meta name=Generator content="Microsoft Word 10 (filtered)">
<style>
<!--
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman";}
a:link, span.MsoHyperlink
{color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{color:purple;
text-decoration:underline;}
p.MsoPlainText, li.MsoPlainText, div.MsoPlainText
{margin:0in;
margin-bottom:.0001pt;
font-size:10.0pt;
font-family:"Courier New";}
@page Section1
{size:8.5in 11.0in;
margin:1.0in 77.95pt 1.0in 77.95pt;}
div.Section1
{page:Section1;}
-->
</style>
</head>
<body lang=EN-US link=blue vlink=purple>
<div class=Section1>
<p class=MsoPlainText style='line-height:150%'><font size=4
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman"'>Hi colleagues,</span></font></p>
<p class=MsoPlainText style='line-height:150%'><font size=4
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman"'> </span></font></p>
<p class=MsoPlainText style='line-height:150%'><font size=4
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman"'>For our image analysis, we need to estimate the
maximum ice thickness in our cryo-EM specimen on 3.5/1 quantifoil grids. We may
be able to estimate that based on the thickness of carbon foil. As indicated by
the quantifoil product catalogue, the standard thickness of carbon foil is 12
nm. While observations of our cryo-EM micrographs have suggested that the
thickness of carbon foil is definitely much larger than 12 nm. Does anybody have
an idea of <font color=blue><span style='color:blue'>what the maximum thickness
of ice could be on quantifoil grids (3.5/1)?</span></font> </span></font></p>
<p class=MsoPlainText style='line-height:150%'><font size=4 color=black
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman";color:black'>Thanks for help!</span></font></p>
<p class=MsoPlainText style='line-height:150%'><font size=4 color=black
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman";color:black'>Guobin Hu</span></font></p>
<p class=MsoPlainText style='line-height:150%'><font size=4 color=black
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
font-family:"Times New Roman";color:black'> </span></font></p>
<p class=MsoNormal><font size=4 face="Times New Roman"><span style='font-size:
14.0pt'> </span></font></p>
</div>
</body>
</html>