[3DEM] How can formvar be removed from holey carbon films?

[Bill Tivol]

On Jan 8, 2004, at 3:24 AM, Koning, R.I. (MCB) wrote:

> Does anybody had any similar experiences and/or has any suggestions or 
> other
> solutions.
>
Dear Roman,
	I have had the same experience, and my solution--only partly 
satidfactory--is to re-do the chloroform-soaked filter paper.  Do not 
let the chloroform liquid pool around the grids; that will tear the 
film.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu