Fwd: convert tld to other format

David Belnap David_Belnap at nih.gov
Tue Jan 14 06:23:46 PST 2003


The format Liang refers to comes from the Z/I (Zeiss) SCAI scanner 
software called Phodis.  Phodis runs on an SGI and controls the SCAI 
scanner.  As far as I can tell, the TLD format is unique to that 
package.  We usually scan images as TLD (so we can use the Phodis 
display and histogram utility).  For storage, we convert the images to 
TIFF with another Phodis utility.  (One can scan directly to TIFF with 
Phodis but then cannot use the Phodis display/histogram utility unless 
the image is converted to TLD.)

Storing Phodis-scanned images in TLD format brings the risks that Liang 
discovered.  It is better to store as TIFF.

David Belnap


Begin forwarded message:

> From: "Philip Koeck" <Philip.Koeck at biosci.ki.se>
> Date: Tue Jan 14, 2003  04:56:34 US/Eastern
> To: "Liang" <tangl at scripps.edu>, <3dem at sdsc.edu>
> Subject: Re: convert tld to other format
>
> I'm not sure whether tld is the same format as our
> Zeiss scanner writes. I'm assuming tld stands for
> 'tiled'.
> Our scanner writes tiled tiff but the files have
> the extension .tif.
>
> We use 'convert' to turn them into sequential tiff.
> for example:
> 'convert image.tif tifs/imagen.tif'
>
> 'convert' should come with any unix installation.
> (At least it works on a DEC)
>
> otherwise try:
> 'tiffcp -s image.tif tifs/image.tif'
>
> Try changing the extension from .tld to .tif
> and then use one of the commands above.
>
> Hope that helps.
>
> Philip Koeck
> S?dert?rns H?gskola and
> Karolinska Institutet
> Dept. of Bioscience at Novum
> S-14157 Huddinge
> Sweden
> phone: +46-8-6089186
> fax: +46-8-6089290
> http://www.biosci.ki.se/em
>
> The phrase 'We have always done things this way.'
> is as much a reason to change as a reason not to.
> - Dartwill Aquila
> _______________________________________
>
>
> ----- Original Message -----
> From: "Liang" <tangl at scripps.edu>
> To: <3dem at sdsc.edu>
> Sent: Tuesday, January 14, 2003 2:44 AM
> Subject: convert tld to other format
>
>
>> Hi, 3D-EMers,
>>
>> Does anybody know if there is a standalone program to convert tld 
>> format
>> to other readable formats such as mrc, tif, pif, etc ? It looks to me
>> EM2EM doesn't do that.
>> I have to reprocess a couple of cryoEM images which were scanned on a
>> Zeiss scanner and stored in tld format. The software for our Zeiss
>> scanner was upgraded and never supports the old, UNIX-based tld 
>> format.
>> And there is nowhere to find the data format of the tld so that one 
>> can
>> write a application to do the conversion.
>>
>> Thanks.
>> Liang
>> -- 
>
>
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>
=============================================
    David M. Belnap
    National Institutes of Health
    50 South Drive (Building 50), Room 1513
    Bethesda, MD  20892-8025  USA

    Tel:  (301) 402-2361
    FAX:  (301) 480-7629
    David_Belnap at nih.gov
=============================================

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