[3dem] Missing metadata .star file during relion_movie_reconstruct

[Ioannis Skalidis]

Dear 3DEM,

I am currently processing two SPA datasets of the same protein and planning
to do a detailed dose-dependent radiation damage comparison so I can
optimize imaging conditions.

To do this, I use RELION 5.1.0 and specifically relion_movie_reconstruct,
after I got everything done, all the way to the final Refine3D after
CTFRefine/Bayesian Polishing of the particles. This is the command I'm
running:

relion_movie_reconstruct --i /home/dose_damage/Refine3D/job063/run_data.star
\
--o recon_attempt_1.mrc \
--movie_angpix 0.513 \
--coord_angpix 0.513 \
--sym C1 \
--corr_mic /home/dose_damage/CtfFind/job007 \
--traj_path /home/dose_damage/Polish/job059 \
--frame 1 \
--ctf true \
--ewald true \
--window 400 \
--scale 400 \
--mask_diameter 200 \
--j 2

Initially, I get an error about the CtfFind version, but it is bypassed:

WARNING: /home/dose_damage/CtfFind/job007 seems to be from a previous
version of Relion. Attempting conversion...
         You should make sure metadata in the optics group table after
conversion is correct.

Then I get the following, at which the process crashes:

ERROR:
Cannot get metadata STAR file for
MotionCorr/job004/data/20260505_145243_dosecheck_Frames/FoilHole_4581204_Data_4580666_4580668_20260505_152108_fractions.mrc

When I check the directory, the .star file for each micrograph is there. Is
it looking for something different? Have I made a mistake in the inputs I
provide to the command? Any advice would be appreciated since it's the
first time I use the specific RELION module.

Cheers,
Ioannis.


Dr. Ioannis Skalidis | Structural Biochemist | Bsky: @johnskal.bsky.social
<https://urldefense.com/v3/__https://bsky.app/profile/johnskal.bsky.social__;!!Mih3wA!GExj8fcVn7fQxEmkVhwIkXXpdWYQ7ieh6MQRyJtiamVDsS3S1ih-JSVKPrDi-G3r48nphYPPwJasog7oX0ANP5Jh$ > | Mobile: +30 698 807 7306 |
johnskalidis at gmail.com
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20260716/16c73e50/attachment.html>