[3dem] Cryo-prep lab humidity and temp control

[Errin Johnson]

Hi all,

We are in the design phase for a new cryo-EM Facility and are currently having discussions with the architects about the temperature and humidity specifications for the cryo-sample prep lab. It would be really useful to benchmark our design against other facilities that have been purpose-built for cryo-EM.

Specifically, it would be great to have info on:


  *   Cryo-sample prep lab dimensions
  *   Set points for both temperature and humidity, plus the tolerance range for each, in the design, what is achieved in practise and how well this is working for cryo-EM grid prep
  *   Whether a push button (or similar) system is used as a de-humidification boost when users are preparing grids, with the humidity conditions relaxing outside these times, and how well this is working in practise

If anyone has gone through the throes of cryo-EM facility design in the last several years and could provide answers to these questions I would very much like to hear from you!

Best wishes,

Errin

Dr Errin Johnson (She/Her)| Bio EM Section Manager
Sydney Microscopy & Microanalysis | Core Research Facilities

THE UNIVERSITY OF SYDNEY
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T +61 2 93519826
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