[3dem] 3dem Digest, Vol 218, Issue 7

Sylvain Trepout sylvain.trepout at monash.edu
Tue Oct 7 03:05:17 PDT 2025 

Dear all,

If you struggle with falcon tubes in your storage dewars because they
float, go outside of the canisters and then sink, just place a stainless
steel nut (M13 is enough) at the bottom of each falcon, they will stay in
the canisters, forever.

Tested and approved for about 15 years.

Best,
Sylvain

PhD
Research Fellow - Microscopy Australia

Monash University

Ramaciotti Centre for Cryo-EM
G92, 15 Innovation Walk, Clayton Campus
Victoria, 3800 Australia

On Tue, 7 Oct 2025, 8:53 pm Ran Zalk via 3dem, <3dem at ncmir.ucsd.edu> wrote:

> Re: Cryo claw
>
> Hi Reza
> I keep a grabber tool in the lab exactly for that purpose. You can find
> one at any Home Depot or on Amazon.
> Cheers
> Ran
>
>
>
> On Mon, Oct 6, 2025 at 9:50 PM <3dem-request at ncmir.ucsd.edu> wrote:
>
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>> Today's Topics:
>>
>>    1. Re: Cryo claw (Rice, William)
>>    2. Re: [External]  Cryo claw (Hamid Rahmani)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Mon, 6 Oct 2025 18:48:44 +0000
>> From: "Rice, William" <William.Rice at nyulangone.org>
>> To: Reza Khayat <rkhayat at ccny.cuny.edu>, 3dem <3dem at ncmir.ucsd.edu>
>> Subject: Re: [3dem] Cryo claw
>> Message-ID:
>>         <
>> DM5PR18MB2149C92D2E62A1E59EC33BAAE4E3A at DM5PR18MB2149.namprd18.prod.outlook.com
>> >
>>
>> Content-Type: text/plain; charset="utf-8"
>>
>> Hi Reza,
>>
>> We have used this tool once to fish out a puck which was dropped in a
>> dewar:
>>
>>
>> https://urldefense.com/v3/__https://www.amleo.com/trash-gator-pick-up-tool-33in/p/GT33?mkwid=*7Cdc&pcrid=&pkw=&pmt=&plc=&kc=&prd=GT33&utm_source=google&utm_term=&utm_campaign=&utm_medium=cpc&slid=&prd=GT33&pgrid=&ptaid=__;JQ!!Mih3wA!DcWQsjSHk_LnVy3cN2p22ZCAkcyatRdiFtZshIS0jlG97mb9IWGZ2lMHWaeX5HrPegstGAkewEabsAteIZCw8OLQG8ci2g$
>>
>>
>> Bill
>>
>> --
>> William Rice
>> Director, Cryo-EM Core Facility
>> Assistant Professor, Cell Biology
>> NYU Grossman School of Medicine
>> Smilow SR C-10
>> 522 First Ave. New York, NY 10016
>> <https://urldefense.com/v3/__https://www.google.com/maps/search/522*First*Ave.*New*York,*NY*10016?entry=gmail&source=g__;KysrKysr!!Mih3wA!BF2WFRaAz-w4ugyId3rWYYHthjOVMtyYSN7wupnXaePWDw6v7iaV4VLAJbfyaA9JolI2rHWT4nYocOK2sIoj4dcTr2mKHICj$ >
>>
>>
>>
>> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Reza Khayat via
>> 3dem <3dem at ncmir.ucsd.edu>
>> Date: Monday, October 6, 2025 at 2:37?PM
>> To: 3dem <3dem at ncmir.ucsd.edu>
>> Subject: [3dem] Cryo claw
>>
>> [EXTERNAL]
>> Hi,
>>
>> We've lost a 50ml falcon tube in our cryo dewar. Is anyone aware of a
>> good claw that can retrieve it? Thanks.
>>
>> Best wishes,
>> Reza
>>
>> Reza Khayat, PhD
>> City College of New York
>> Department of Chemistry and Biochemistry
>>
>>
>>
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>> ------------------------------
>>
>> Message: 2
>> Date: Mon, 6 Oct 2025 18:49:53 +0000
>> From: Hamid Rahmani <hrahmani at scripps.edu>
>> To: "Brilot, Axel" <axel.brilot at austin.utexas.edu>, 3dem
>>         <3dem at ncmir.ucsd.edu>, Reza Khayat <rkhayat at ccny.cuny.edu>,
>> "Morgan,
>>         David Gene" <dagmorga at iu.edu>
>> Subject: Re: [3dem] [External]  Cryo claw
>> Message-ID:
>>         <
>> IA1PR11MB62201466A39002368EDC2288C0E3A at IA1PR11MB6220.namprd11.prod.outlook.com
>> >
>>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Hi all, we have one of these (attached) and I have retrieved a puck with
>> it before. However, I got lucky and did it without looking!
>>
>> Best,
>> Hamid
>> ________________________________
>> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Brilot, Axel via
>> 3dem <3dem at ncmir.ucsd.edu>
>> Sent: Monday, October 6, 2025 11:45:02 AM
>> To: 3dem <3dem at ncmir.ucsd.edu>; Reza Khayat <rkhayat at ccny.cuny.edu>;
>> Morgan, David Gene <dagmorga at iu.edu>
>> Subject: Re: [3dem] [External] Cryo claw
>>
>> Hi Reza, David,
>>
>> Alternatively, you can buy very long crucible tongs (~ 20 inches or
>> longer) and hope to get lucky. If you empty out all the cylinders from the
>> dewar and wait for a few minutes you should be able to get a good view with
>> a flashlight to find its location and get it out.
>>
>> HTH,
>>
>> Axel
>>
>> ________________________________
>> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Morgan, David Gene
>> via 3dem <3dem at ncmir.ucsd.edu>
>> Sent: Monday, October 6, 2025 1:41 PM
>> To: 3dem <3dem at ncmir.ucsd.edu>; Reza Khayat <rkhayat at ccny.cuny.edu>
>> Subject: Re: [3dem] [External] Cryo claw
>>
>> Reza,
>>
>> Your best bet is probably to empty the entire dewar.  Large styrofoam
>> boxes are very good for this sort of thing...
>>
>> --
>>
>> NOTE:  my email has changed to dagmorga at iu.edu.
>>              Email to dagmorga at indiana.edu will stop being
>>              forwarded later this year.
>>
>>     politics is more difficult than physics.
>>                                              A. Einstein
>>
>>             David Gene Morgan
>>         Electron Microscopy Center
>>              043E Simon Hall
>>              IU Bloomington
>>           812 856 1457 (office)
>>           812 856 3221 (3200)
>>
>> https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!EhQko7SNLFXBm4NXTqseCZZ2K1wkC1EYMZCU3ReDyj136V-OFcvdJUQNRHiBdnyejeMg46EOt9eyheDRPFA4q_4$
>> <
>> https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!E_ZCk5nwVmid3Ld1RYwqrrMqgoDimX50bFu-FtpO3HyhPFK9M6szfCMmMSnIxwTA7rEOr3kiu0SMYaDrh8Y$
>> >
>> ________________________________
>> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Reza Khayat via
>> 3dem <3dem at ncmir.ucsd.edu>
>> Sent: Monday, October 6, 2025 2:36 PM
>> To: 3dem <3dem at ncmir.ucsd.edu>
>> Subject: [External] [3dem] Cryo claw
>>
>>
>> This message was sent from a non-IU address. Please exercise caution when
>> clicking links or opening attachments from external sources.
>>
>>
>> Hi,
>>
>> We've lost a 50ml falcon tube in our cryo dewar. Is anyone aware of a
>> good claw that can retrieve it? Thanks.
>>
>> Best wishes,
>> Reza
>>
>> Reza Khayat, PhD
>> City College of New York
>> Department of Chemistry and Biochemistry
>>
>>
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>>
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>> ------------------------------
>>
>> End of 3dem Digest, Vol 218, Issue 7
>> ************************************
>>
>
>
> --
> Ran Zalk, Ph.D
> Associate Research Scientist
> National Institute for Biotechnology in the Negev
> Ben-Gurion University of the Negev
> Building 39, rm. 005
> Beer Sheva 84105, Israel
> Office: 972-8-647-9197
> _______________________________________________
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