[3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols

[Gibson, Kimberley]

Hi Jason,

Typically, at BSL-2, all equipment can be operated under normal conditions - i.e. in the open on a lab bench (Vitrobot or Leica  GPS) with special considerations for the evacuation of N2 gas or Ethane and Ethane/Propane mixtures.

Issues regarding BSL-2 have only arisen during disposal of equipment. TFS was not allowed to ship an old Ion Getter Pump overseas (from the US back to Eindhoven, NL) if the microscope operated in a BLS-2 lab. The IGP had to be disposed of locally and typically sprayed down with Ethanol prior to disposal. I have also sprayed an old Peltier device removed from a Vitrobot and sent it for electronic waste disposal.

Best,

Kim

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Matthijn VOS via 3dem <3dem at ncmir.ucsd.edu>
Sent: Thursday, June 12, 2025 06:32
To: Jason NG <jasonng.cpos at hku.hk>
Cc: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols

Dear Jason,

could you explain the physics hiw sn electron beam can create aerosols on a solid sample ar -196 degrees in ultra high vacuum (orders of magnitude under the tripple point of water)

I don’t see how this is possible.

cheers

Matthijn

On Jun 12, 2025, at 11:01, Jason NG (CPOS) via 3dem <3dem at ncmir.ucsd.edu> wrote:



Dear CryoEM Community,



We are currently evaluating the risks of becoming BSL-2 CryoEM laboratory and would greatly appreciate your guidance on several safety and setup considerations.



Specifically, we are interested in understanding:

  *   Given that the electron beam can potentially generate aerosols or cause warming within TEMs, what disinfection protocols or measures have you found effective for maintaining biosafety?
  *   Is implementing negative room pressure necessary in a CryoEM lab at BSL-2?
  *   For sample preparation instruments such as the TFS Vitrobot and Leica GP2, is it recommended to place them inside a biosafety cabinet? How about large instruments like the Leica ICE—are there specific safety measures we should consider?



Your insights and experiences would be extremely helpful as we evaluate the best practices for creating a safe and effective CryoEM environment. Thank you very much for your time and guidance.



Best Regards,

Jason NG

Senior Technical Assistant

LKS Cryo-EM Laboratory



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Centre for PanorOmic Sciences

LLG03, Laboratory Block

21 Sassoon Road, Pokfulam, Hong Kong

P: +852 3910-3528

E: jasonng.cpos at hku.hk<mailto:jasonng.cpos at hku.hk>

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