[3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols

[Jason NG (CPOS)]

Dear CryoEM Community,

We are currently evaluating the risks of becoming BSL-2 CryoEM laboratory and would greatly appreciate your guidance on several safety and setup considerations.

Specifically, we are interested in understanding:

  *   Given that the electron beam can potentially generate aerosols or cause warming within TEMs, what disinfection protocols or measures have you found effective for maintaining biosafety?
  *   Is implementing negative room pressure necessary in a CryoEM lab at BSL-2?
  *   For sample preparation instruments such as the TFS Vitrobot and Leica GP2, is it recommended to place them inside a biosafety cabinet? How about large instruments like the Leica ICE-are there specific safety measures we should consider?

Your insights and experiences would be extremely helpful as we evaluate the best practices for creating a safe and effective CryoEM environment. Thank you very much for your time and guidance.

Best Regards,
Jason NG
Senior Technical Assistant
LKS Cryo-EM Laboratory

[cid:image001.jpg at 01DBDBB8.458D38B0]

Centre for PanorOmic Sciences
LLG03, Laboratory Block
21 Sassoon Road, Pokfulam, Hong Kong
P: +852 3910-3528
E: jasonng.cpos at hku.hk<mailto:jasonng.cpos at hku.hk>
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