[3dem] 3dem Digest, Vol 204, Issue 11

[David Bulkley]

It seems like humidity plays poorly with filter paper in the GP2 for most
folks.  We typically just leave our humidifier off when we are blotting
cells.  Is this a bad idea?  We've been able to get some decent lamella
from cells blotted with the chamber humidifier off, but I'm curious if
folks have done any proper testing on this to verify if the cells survive,
and if they are damaged, what the symptoms are.  It would suck to find out
that, while my filter paper has been doing great, all of my cells have been
getting trashed.  Curious if anyone has some real data on this.

On Fri, Aug 9, 2024 at 12:00 PM <3dem-request at ncmir.ucsd.edu> wrote:

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>    1. Re: Inconsistent Blotting with GP2 (Sen, Anindito)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Fri, 9 Aug 2024 17:14:26 +0000
> From: "Sen, Anindito" <andysen at tamu.edu>
> To: Liz Montabana <liz.montabana at czii.org>, "3dem at ncmir.ucsd.edu"
>         <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Inconsistent Blotting with GP2
> Message-ID:
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> SN6PR11MB3213B188B5CE3D8FBBCACAE0C7BA2 at SN6PR11MB3213.namprd11.prod.outlook.com
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> HI Liz,
>
> Is the chamber humidity too high (the filter papers get wet?).
>
> Stay safe & Regards
>
> Anindito Sen. Ph.D
>
> Microscopy and Imaging Center
> Texas A&M University
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> College Station, TX 77843-2257
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> Office: ILSB Room 1133
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> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Liz Montabana <
> liz.montabana at czii.org>
> Date: Wednesday, August 7, 2024 at 6:59 PM
> To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Inconsistent Blotting with GP2
> Hi All We are seeing a lot of inconsistent blotting with our GP2 plunge
> freezer (The longer we blot, the thicker our grids are, among other odd
> behavior) - and we wanted to ask others' experience to see if we could
> pinpoint any cause or
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> Hi All
>
> We are seeing a lot of inconsistent blotting with our GP2 plunge freezer
> (The longer we blot, the thicker our grids are, among other odd behavior) -
> and we wanted to ask others' experience to see if we could pinpoint any
> cause or feedback we could provide Leica to enhance the performance.
>
> Please share your trade tips and secrets for wrangling the GP2 - We've had
> experience with several other GP2s and none of them shared this behavior.
> Our main samples are whole cells grown on grids.
>
> Thanks
> Liz Montabana
> Chan Zuckerberg Imaging Institute
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