[3dem] Graphene Oxide grids hydrophobic

[Farzad]

Desr Dario,

GO looses it's oxygens and other polar functional groups under light (even
IR) and turns into normal graphene. It must be stored under dark and dry
condition.

Have a nice weekend.

Kindest regards

Ferrie



Dr.-Ing Farzad Hamdi,
EM Engineer - Staff Scientist
Kastritis Laboratory for Biomolecular Research (KLBR)
Cryo-Electron Microscopy & Computational Structural Biology
__
Institute of Biochemistry and Biotechnology
Martin-Luther-Universität Halle-Wittenberg (MLU)
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On Thu, Dec 7, 2023, 17:48 Dario <dariobrack at googlemail.com> wrote:

> Dear all,
>
> I have ordered commercial GO grids (QF 300 Cu R2/2 GO) and expected them
> to be hydrophilic out of the box due to the GO layer.
> However, the grid was not covered by the 3ul sample drop and appeared
> quite hydrophobic. How do you prepare the GO grids for plunging?
>
> I plasma cleaned them with air for 8sec, after that they were very
> hydrophilic, but I am afraid I might have destroyed the GO layer.
>
> Best
>
> Dario
>
>
>
>
> _______________________________
>
> Dr. Dario Saczko-Brack
> Postdoc
>
> Ludwig-Maximilians-Universität München
> Department of Cellular Physiology
> Biomedical Center Munich - BMC
> Großhaderner Str. 9
> D-82152 Planegg-Martinsried
>
> Prof. Dr. Claudia Veigel
>
> Room: N.C 01.033
> Phone: +49 (0) 89 2180 71588
> Fax: +49 (0) 89 2180 71732
> Email: Dario.Saczko-Brack at med.lmu.de
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>
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