[3dem] Graphene Oxide grids hydrophobic
Farzad
farzaad at gmail.com
Fri Dec 8 08:39:54 PST 2023
Desr Dario,
GO looses it's oxygens and other polar functional groups under light (even
IR) and turns into normal graphene. It must be stored under dark and dry
condition.
Have a nice weekend.
Kindest regards
Ferrie
Dr.-Ing Farzad Hamdi,
EM Engineer - Staff Scientist
Kastritis Laboratory for Biomolecular Research (KLBR)
Cryo-Electron Microscopy & Computational Structural Biology
__
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On Thu, Dec 7, 2023, 17:48 Dario <dariobrack at googlemail.com> wrote:
> Dear all,
>
> I have ordered commercial GO grids (QF 300 Cu R2/2 GO) and expected them
> to be hydrophilic out of the box due to the GO layer.
> However, the grid was not covered by the 3ul sample drop and appeared
> quite hydrophobic. How do you prepare the GO grids for plunging?
>
> I plasma cleaned them with air for 8sec, after that they were very
> hydrophilic, but I am afraid I might have destroyed the GO layer.
>
> Best
>
> Dario
>
>
>
>
> _______________________________
>
> Dr. Dario Saczko-Brack
> Postdoc
>
> Ludwig-Maximilians-Universität München
> Department of Cellular Physiology
> Biomedical Center Munich - BMC
> Großhaderner Str. 9
> D-82152 Planegg-Martinsried
>
> Prof. Dr. Claudia Veigel
>
> Room: N.C 01.033
> Phone: +49 (0) 89 2180 71588
> Fax: +49 (0) 89 2180 71732
> Email: Dario.Saczko-Brack at med.lmu.de
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>
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