[3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids

[David Michael Belnap]

Guobin,

I have used 671-200-Cu grids from Ted Pella several times without problems.  I don’t recall ever having drift problems.  I was imaging virus or virus-like particles on these grids.  671-200-Cu grids are Quantifoil R2/1 grids coated with 2 nm C.

David

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David M. Belnap
Electron Microscopy Core Laboratory
Department of Biochemistry
School of Biological Sciences
2501 SMBB
University of Utah
Salt Lake City, Utah 84112 USA
Phone  801.585.1242
FAX  801.587.3077
David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>
=======================================



From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Guobin Hu <gb_hu at yahoo.com>
Date: Friday, November 18, 2022 at 8:22 PM
To: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
Subject: [3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids

Dear all,

I am collecting data with 2nm ultrathin carbon coated Quantifoil holy grids and observing excessive/abnormal drift/motion in images which I did not see with regular Quantifoil grids or other type of grids. There is no indication of the microscope as the cause of drift/motion. I am curious if anyone else has also observed the similar motion/drift with 2nm ultrathin carbon coated grids. FFTs w/ and w/o  drift/motion are attached as references. Any feedback insight will be appreciated.

Thanks,
Guobin




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