[3dem] eBIC EM scientist/Senior EM scientist
peijun at strubi.ox.ac.uk
peijun at strubi.ox.ac.uk
Tue Jan 26 16:19:19 PST 2021
Apologize for the error in the subject line in my previous post.
The eBIC position is not a Postdoc position, but a permanent EM Scientist/Senior EM Scientist position.
Best,
Peijun
------------------------------------------------------------
Date: Tue, 26 Jan 2021 20:17:33 -0000
From: peijun at STRUBI.OX.AC.UK
Subject: Re: Postdoc position open in eBIC, Diamond Light Source, UK
Dear all,
I would like to bring to your attention an EM scientist/senior scientist position (full time/permanent) in eBIC, Diamond Light Source <https://urldefense.com/v3/__https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC.html__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxzX5duJAg$ > .
Please the link below for the vacancy.
https://urldefense.com/v3/__https://vacancies.diamond.ac.uk/vacancy/cryoelectron-microscopy-scientist-se__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxwy6H9LtQ$
nior-scientist-429428.html
Best,
Peijun
Peijun Zhang
Professor
Division of Structural Biology
Nuffield Department of Medicine
University of Oxford
Roosevelt Drive Oxford OX3 7BN, UK
<mailto:peijun at strubi.ox.ac.uk> peijun at strubi.ox.ac.uk
<https://urldefense.com/v3/__https://ndm.medsci.ox.ac.uk/people/peijun-zhang__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxy08tTSeQ$ >
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Director
Electron Bio-Imaging Centre (eBIC)
Diamond Light Source
<https://urldefense.com/v3/__https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC.html__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxzX5duJAg$ >
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Date: Tue, 26 Jan 2021 21:45:48 +0100
From: Sonja Lorenz <sonja.lorenz at VIRCHOW.UNI-WUERZBURG.DE>
Subject: Postdoctoral position in cryo-EM at the Max Planck Institute for Biophysical Chemistry, Goettingen
Dear colleagues,
there is a postdoctoral position available in my laboratory at the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany.
We are looking for a curiosity-driven colleague to explore ubiquitin ligase complexes by cryo-EM.
For details, please see:
https://urldefense.com/v3/__https://www.mpibpc.mpg.de/17641695/04-21__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxwyu2RSXQ$
I would be grateful if you could make this announcement available to candidates at your institutes.
Many thanks and best wishes,
Sonja Lorenz, PhD
Max Planck Institute for Biophysical Chemistry Research Group „Ubiquitin Signaling Specificity“ Am Fassberg 11
37077 Göttingen
Germany
Web: https://urldefense.com/v3/__http://www.mpibpc.mpg.de/lorenz__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxyXxsRUUQ$
Twitter @SLorenzLab
==========================
The Max Planck Institute for Biophysical Chemistry is one of the largest institutes of the Max Planck Society for the Advancement of Science and conducts basic research to advance knowledge and benefit society. Innovative projects and interdisciplinary cooperation characterize research within the Max Planck Society.
The research group Ubiquitin Signaling Specificity (Sonja Lorenz) invites applications for a position as Postdoc (f/m/div.) – Structural and functional analyses of ubiquitin ligase complexes –
Our lab aims to understand how ubiquitin − a single, small protein − achieves specificity in regulating virtually all aspects of eukaryotic cell biology. A major key lies in the action of ubiquitin ligases, the most diversified class of enzymes of the ubiquitination machinery. The immense potential of ubiquitin ligases as therapeutic targets is illustrated by the clinical efficacy of thalidomide and derivatives in the treatment of hematologic malignancies. However, progress towards manipulating ubiquitin ligases rationally has been impeded largely by our insufficient understanding of their structural underpinnings and functional integration into cellular pathways.
Our lab has establied dedicated tools to capture dynamic macromolecular complexes of ubiquitination enzymes and decipher the molecular basis of their specificities in substrate recognition and ubiquitin linkage formation. We combine chemical-biological, biochemical, cell biological, and structural approaches with a particular focus on exploiting the power of cryo-electron microscopy to resolve large macromolecular assemblies.
We have a fully funded postdoc position available for a talented individual to explore critical ubiquitin ligase complexes in infectious disease or tumor biology.
Your profile
• You have a PhD or equivalent degree in a relevant subject area, such as structural biology, biochemistry or biophysics.
• You have a proven track record and experience in cryo-EM and recombinant protein preparation, ideally using the baculovirus expression system.
• Additional experience in X-ray crystallography would be beneficial.
• You are curiosity-driven and passionate about science, and not afraid of tackling challenging protein complexes.
• You are self-motivated and independent, and enjoy being part of an international and multidisciplinary work environment.
About us
We are an independent research group at the Max Planck Institute for Biophysical Chemistry, one of Germany’s premier research campuses with leading-edge infracture in all fields of structural and cell biology. We are a highly international and interdisciplinary team, funded by the Max Planck Society and the German Research foundation. The historic city of Goettingen, located in the center of Germany, offers great outdoor and cultural opportunities, a bustling student scene, and an impressive scientific heritage.
Position details
The position should be filled as soon as possible, but the exact start date is flexible. The position is initially for 2 years with the possibility of extension. Payment and benefits are based on the TVöD guidelines.
The Max Planck Society is committed to increasing the number of individuals with disabilities in its workforce and therefore encourages applications from such qualified individuals.
Application
Please submit your application including cover letter (explaining background and motivation), CV, transcripts, and publication record by e-mail as a single PDF file to ausschreibung04-21 at mpibpc.mpg.de
Max Planck Institute for Biophysical Chemistry Research Group „Ubiquitin Signaling Specificity“ Sonja Lorenz Am Fassberg 11
37077 Göttingen
Germany
Web: https://urldefense.com/v3/__http://www.mpibpc.mpg.de/lorenz__;!!Mih3wA!WHKijwc_d_VtdEg8-t5Fb66qmqnHV8NlLSkQK_OFXQDMiv56yLqEBtytoxyXxsRUUQ$
Twitter @SLorenzLab
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------------------------------
Date: Tue, 26 Jan 2021 21:05:34 +0000
From: "Liu, Lan (NIH/NIDDK) [F]" <lan.liu at NIH.GOV>
Subject: Re: Problem on grids coated with continuous carbon film
Dear Yanxiang,
Thank you for your reply. I use TF20 to inspect grids. I usually check 4-8 grids with the same holder per day and make sure the stage is free of obvious ice contamination. There is no ice ring or spots on the CTF image of these dirty micrographs, so I think they may not be the ice contamination. Do you think we may introduce other types of contaminations during grids loading?
Best regards,
Lan
From: YANXIANG CUI <yanxiangcui at ucla.edu>
Date: Tuesday, January 26, 2021 at 3:48 PM
To: Liu, Lan (NIH/NIDDK) [F] <lan.liu at nih.gov>
Subject: Re: [ccpem] Problem on grids coated with continuous carbon film Hi Lan,
Which microscope do you use to screen the frozen grids, did you check the holder condition before using it if you use a side-insert holder? It looks like the grid has already been polluted by dirty ice, maybe because the temperature increased during the grid loading.
In my opinion, I dont' think it's related to the coating.
Best regards
Yanxiang Cui
On Tue, Jan 26, 2021 at 11:38 AM Liu, Lan (NIH/NIDDK) [F] <000055685c494465-dmarc-request at jiscmail.ac.uk<mailto:000055685c494465-dmarc-request at jiscmail.ac.uk>> wrote:
Dear all,
Recently I encountered a problem on grids coated with continuous carbon film.
The continuous carbon film (3~4 nM) was made by Leica EM ACE600 and coated on C-flat and Quantifoil grids. When I used these grids, sometime I could get a clean grid and collected a good data, whereas sometimes the grids were so dirty that I couldn’t see any particles. Even grids which were made in the same day with the same condition had different quality. The ratio of the good grids is only about 30%. I made several batches of carbon film grids and this problem repeatedly happened.
I checked the grids on Tecnai 12 before freezing. The grids before or after carbon film coating are clean. Does the grids become “dirty” during freezing? Previously I used grids without continuous carbon film and only recently used the continuous carbon film grids. I wonder if there is any special tips to make and use carbon film grids.
I listed some “dirty” examples in the attached file. Any suggestions and comments are appreciated.
Thank you.
Best regards,
Lan
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------------------------------
Date: Tue, 26 Jan 2021 13:11:04 -0800
From: YANXIANG CUI <yanxiangcui at UCLA.EDU>
Subject: Re: Problem on grids coated with continuous carbon film
Hi Lan,
Those images are very common when the vacuum broken during grid loading.
Another thing we need to consider is the ethane, did you change the ethane recently?
Best regards
Yanxiang cui
On Tue, Jan 26, 2021 at 1:06 PM Liu, Lan (NIH/NIDDK) [F] <lan.liu at nih.gov>
wrote:
> Dear Yanxiang,
>
>
>
> Thank you for your reply. I use TF20 to inspect grids. I usually check
> 4-8 grids with the same holder per day and make sure the stage is free
> of obvious ice contamination. There is no ice ring or spots on the
> CTF image of these dirty micrographs, so I think they may not be the
> ice contamination. Do you think we may introduce other types of
> contaminations during grids loading?
>
>
>
> Best regards,
>
> Lan
>
>
>
>
>
> *From: *YANXIANG CUI <yanxiangcui at ucla.edu>
> *Date: *Tuesday, January 26, 2021 at 3:48 PM
> *To: *Liu, Lan (NIH/NIDDK) [F] <lan.liu at nih.gov>
> *Subject: *Re: [ccpem] Problem on grids coated with continuous carbon
> film
>
> Hi Lan,
>
>
>
> Which microscope do you use to screen the frozen grids, did you check
> the holder condition before using it if you use a side-insert holder?
> It looks like the grid has already been polluted by dirty ice, maybe
> because the temperature increased during the grid loading.
>
>
>
> In my opinion, I dont' think it's related to the coating.
>
>
>
> Best regards
>
> Yanxiang Cui
>
>
>
> On Tue, Jan 26, 2021 at 11:38 AM Liu, Lan (NIH/NIDDK) [F] <
> 000055685c494465-dmarc-request at jiscmail.ac.uk> wrote:
>
> Dear all,
>
>
> Recently I encountered a problem on grids coated with continuous
> carbon film. The continuous carbon film (3~4 nM) was made by Leica EM
> ACE600 and coated on C-flat and Quantifoil grids. When I used these
> grids, sometime I could get a clean grid and collected a good data,
> whereas sometimes the grids were so dirty that I couldn’t see any
> particles. Even grids which were made in the same day with the same condition had different quality.
> The ratio of the good grids is only about 30%. I made several batches
> of carbon film grids and this problem repeatedly happened. I checked
> the grids on Tecnai 12 before freezing. The grids before or after
> carbon film coating are clean. Does the grids become “dirty” during freezing?
> Previously I used grids without continuous carbon film and only
> recently used the continuous carbon film grids. I wonder if there is
> any special tips to make and use carbon film grids. I listed some
> “dirty” examples in the attached file. Any suggestions and comments are appreciated. Thank
> you. Best regards, Lan
>
>
> ------------------------------
>
> To unsubscribe from the CCPEM list, click the following link:
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> 1
>
>
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