[3dem] using MTF during framealignemnt

[Takanori Nakane]

Hi,

It does not matter. MTF only changes the power spectrum but not the resolution.

Best regards,

Takanori Nakane

On 2020/09/08 21:30, Jay Rai wrote:
> Actually I want to ask something like this..
> Relion3.1 uses the MTF of the detector during refinement stages of refinement. So that which MTF of the detector should give the better results -CDS 
> or standard ? Anyone has any idea!
> 
> With kind regards,
> 
> Jay Rai​
> 
> ------------------------------------------------------------------------------------------------------------------------------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Jay Rai <jrai at fsu.edu>
> *Sent:* Tuesday, September 8, 2020 4:16 PM
> *To:* Takanori Nakane <tnakane at mrc-lmb.cam.ac.uk>; 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* Re: [3dem] using MTF during framealignemnt
> Thank you for the answer. The question was wrong. I was thinking of something else. But I did understand what you mean?
> 
> With kind regards,
> 
> Jay Rai​
> 
> ------------------------------------------------------------------------------------------------------------------------------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Takanori Nakane <tnakane at mrc-lmb.cam.ac.uk>
> *Sent:* Tuesday, September 8, 2020 3:46 PM
> *To:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* Re: [3dem] using MTF during framealignemnt
> Hi,
> 
> What do you mean by framealignment? Motion correction or Bayesian Polishing?
> 
> Both don't use an MTF file.
> 
> Best regards,
> 
> Takanori Nakane
> 
> On 2020/09/08 20:31, Jay Rai wrote:
>> Hi EM community,
>> Has anyone ever use MTF.star file during framealignement in relion 3.1? If so then which MTF is better, CDS mode or standard mode (K3 camera)?
>> I am talking about the frame alignment of normal counting data.
>> Any comments or suggestion are highly welcome.
>> 
>> With kind regards,
>> 
>> Jay Rai​
>> 
>> 
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