[3dem] Pre-multiplied CTF option in Relion
Takanori Nakane
tnakane at mrc-lmb.cam.ac.uk
Tue Sep 1 23:25:15 PDT 2020
Hi,
It should work in 3.1.0.
Apply it in Polishing with --ctf_premultiply and --crop.
See https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind1912&L=CCPEM&O=D&P=42487__;!!Mih3wA!StZGac4eSCCvJReP9WnDgop6znVaGeGORR_tIcejXQKHpa2Man3cCLNDD7r9VmdySg$ for details.
Also note that there are several limitations.
https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind2008&L=CCPEM&O=D&P=103727__;!!Mih3wA!StZGac4eSCCvJReP9WnDgop6znVaGeGORR_tIcejXQKHpa2Man3cCLNDD7q5NFM2Zw$
Best regards,
Takanori Nakane
On 2020/09/02 1:40, Craig Yoshioka wrote:
> Hi all,
>
> Does anyone have much experience with using pre-multiplied CTFs in Relion? When I last tried it, it was not supported in the GPU branch, but I was interested in seeing if I could use it to get around CTF-aliasing and delocalization without using huge box sizes (with the assumption that the pre-multiplication occurs before particle windowing).
>
> Cheers,
> -Craig
>
>
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