[3dem] About dynamics
Carlos Oscar Sorzano
coss at cnb.csic.es
Tue May 26 02:21:24 PDT 2020
Dear Jay,
what we have observed is that image classification can be, especially
for small particles, a very unstable process, no matter which program
you use. You may even call the same program twice having remarkably
different results. What we are promoting is the use of "consensus"
classification: two particles go together in the same class if the two
particles have been consistently put together in multiple runs of the
same program, or even better, by different algorithms. We only believe
those classification results that have been replicated independently.
In Scipion there are easy ways to compute these intersections of sets,
but I am sure that this can also be performed in other programs.
Kind regards, Carlos Oscar
On 5/26/20 2:03 AM, Jay Rai wrote:
> Just to be add: Its work like a charm on big complexes like ribosome.
> In other words- which software is best for the multibody refinement/
> protein dynamics?
> Thanking you in advance,
> jay
> ------------------------------------------------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Jay Rai
> <jrai at fsu.edu>
> *Sent:* Monday, May 25, 2020 3:27 PM
> *To:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* [3dem] About dynamics
> Dear Cryo-EM folks,
> I am trying to solve the dynamics of 300 kD multi proteins complex.
> Any idea which software is the most useful? Any tips and suggestions.
> So, far I tried Relion and cryosprac. Both gave me different results.
> Thanks
> Jay
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20200526/6cffba4f/attachment.html>
More information about the 3dem
mailing list