[3dem] published B factors
Alexis Rohou
a.rohou at gmail.com
Sat Aug 29 16:20:40 PDT 2020
Dear colleagues,
Thanks to those who replied. Below are a few examples I gathered of
structures for which both types of B factors were available.
Cheers,
Alexis
B factor (Å2)
Estimation method
Reference
In situ bacterial 70S ribosomal subunit at 3.7 Å
5
Amplitude spectrum
(Tegunov *et al.*, 2020) and pers. comm. from D. Tegunov
86
ResLog
Mouse apoferritin at 1.22 Å
2.3
Amplitude spectrum
(Nakane *et al.*, 2020)
32.5
ResLog
Urease at 1.98 Å
38
Amplitude spectrum
(Righetto *et al.*, 2020) and pers. comm. from R. Righetto
64.1
ResLog
Apoferritin at 1.96 Å
50
Amplitude spectrum
Pers. comm. from R. Righetto
74
ResLog
Mycobacterial secretion system ESX-3 dimer at 3.8 Å
101
Amplitude spectrum
(Famelis *et al.*, 2019) and pers. comm. from A. Rivera Calzada
144
ResLog
Famelis, N. *et al.* (2019) ‘Architecture of the mycobacterial type VII
secretion system.’, *Nature*, 576(7786), pp. 321–325. doi:
10.1038/s41586-019-1633-1.
Merk, A. *et al.* (2020) ‘1.8 Å resolution structure of β-galactosidase
with a 200 kV CRYO ARM electron microscope’, *IUCrJ*. International Union
of Crystallography, 7(4), pp. 1–5. doi: 10.1107/s2052252520006855.
Nakane, T. *et al.* (2020) ‘Single-particle cryo-EM at atomic resolution’.
Righetto, R. D. *et al.* (2020) ‘High-resolution cryo-EM structure of
urease from the pathogen Yersinia enterocolitica’, *bioRxiv*, p.
2020.04.28.065599. doi: 10.1101/2020.04.28.065599.
Tegunov, D. *et al.* (2020) ‘Multi-particle cryo-EM refinement with M
visualizes ribosome-antibiotic complex at 3 . 7 Å inside cells’.
On Sun, Jul 5, 2020 at 11:49 AM Alexis Rohou <a.rohou at gmail.com> wrote:
> Dear colleagues,
>
> If you know of a publication that includes, for the same dataset, both:
>
> (1) an estimate of the "sharpening" B factor (i.e. based on the radial
> amplitude spectrum of a 3D reconstruction) as well as
> (2) the B factor estimated from a plot of resolution as a function of
> number of particles (also known as ResLog plot),
>
> ... would you please point me to relevant citation(s)?
>
> Thanks!
> Alexis
>
> PS. Feel free to reply to me directly if you prefer - I can summarize
> findings to the list in a few days
>
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