[3dem] 3D classification
Eric Hanssen
ehanssen at unimelb.edu.au
Thu Jul 11 17:23:31 PDT 2019
Hi all,
Before I commit some computer resource to a job I'd rather know if it is doable.
I have 200,000 particle of a 600kDa protein, a current map at 3.4A that looks very nice, I do not need a better resolution for now. I have a drug (dipeptide) attached to the protein but only a subset of the protein has the drug bound. Is it possible with a 3D classification to separate such a small difference? I don't mind which software, relion, cryosparc, eman2 ....
Or is there a better way, eg. 3D classification of a very small box size centered around the binding site?
Cheers
Eric
-----------------
Assoc. Prof Eric Hanssen
Head - Advanced Microscopy Facility
Honorary Principal Fellow - Department of Biochemistry and Molecular Biology
President Australian Microscopy and Microanalysis Society
Bio21 Molecular Science and Biotechnology Institute
30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
email: ehanssen at unimelb.edu.au<mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509
Web: www.microscopy.unimelb.edu.au<http://www.microscopy.unimelb.edu.au/>
[bio21] [amms-logo]
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