[3dem] Recommendation of CTF estimation program

[Guobin Hu]

 Thanks again Carlos.--Guobin
    On Friday, February 15, 2019, 2:06:02 AM EST, Carlos Oscar S. Sorzano <coss at cnb.csic.es> wrote:  
 
  
Remind that the best way is to use Xmipp through Scipion.
 
Kind regards, Carlos Oscar
 
 El 15/02/2019 a las 0:08, Guobin Hu escribió:
  
 
 Dear Carlos, 
  Thank you very much for your feedback and suggestion. Answering your question. Yes. I see Thon rings in FFT. I would check out Xmipp.  
  Best, Guobin
 
 Sent from my iPhone, please excuse typos 
 On Feb 14, 2019, at 5:07 PM, Carlos Oscar S. Sorzano <coss at cnb.csic.es> wrote:
 
  
  

Dear Guobin,
 
stated like this, it is to difficult to give an "absolute" recommendation. A first question would be if you see the Thon rings by eye? If not, I would say that most programs will not be able to find the defocus. If you can see them, then it is a matter of trying. All I can offer in this regard is to try Xmipp on this, if you make one or two of these micrographs available, you may send me a personal message with the link and we will check them.
 
Kind regards, Carlos Oscar
 
 El 14/02/2019 a las 21:40, Guobin Hu escribió:
  
 
Dear Cryo-EM folks, 
  I am dealing with some micrographs of very low defocus values e.g. a few tens of nanometers. I found it sometimes is difficult for the programs I am using to find the right defocus value even I set the corresponding defocus search range. I see a lot of CTF estimation programs on the wikipage. I do not want to download all of them and try one by one. I would greatly appreciate it if somebody can recommend one that works  well with lowly defocused micrographs. The best is the program can also display the Thon rings of CTF of very small defocus values.  
  Best, Guobin Hu  
  _______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
 -- 
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano                  e-mail:   coss at cnb.csic.es
Biocomputing unit                             http://i2pc.es/coss
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco)     Tlf: 34-91-585 4510
28049 MADRID (SPAIN)                          Fax: 34-91-585 4506
------------------------------------------------------------------------
 
 _______________________________________________
 3dem mailing list
 3dem at ncmir.ucsd.edu
 https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
  
 -- 
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano                  e-mail:   coss at cnb.csic.es
Biocomputing unit                             http://i2pc.es/coss
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco)     Tlf: 34-91-585 4510
28049 MADRID (SPAIN)                          Fax: 34-91-585 4506
------------------------------------------------------------------------
   
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20190215/08651dcb/attachment.html>