[3dem] freezing conditions in high salt?
frangube
frangube at gmail.com
Mon Sep 3 02:55:37 PDT 2018
Dear all,
I was wondering if anyone has experience in obtaining nice, thin ice by freezing a protein in 300mM NaCl (and 20mM Hepes pH 8, no detergent) using a Leica EM GP.
If anyone has suggestions I would really appreciate it!
Thanks in advance.
Francesca
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Dr Francesca Gubellini
Unité Microbiologie Structurale
Institut Pasteur
25 rue du Docteur Roux
75015 Paris
France
Tel: +33 (0)1.40.61.35.31
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