[3dem] [ccpem] 2D centering of particle images
Marin van Heel
marin.vanheel at googlemail.com
Tue Jul 24 03:34:20 PDT 2018
Dear Steven Wang,
Alignment is more of a statistical issue affecting the whole data set
than something that should be done on an individual (noisy)
particle-by-particle basis.
See, for example, our: "Alignment By Classification" (ABC) paper:
http://journals.iucr.org/m/issues/2017/05/00/kf5002/index.html
But our IMAGIC-4D software does of course have various options to do it
particle-by-particle if that is what you insist you need for testing.
Cheers
Marin van Heel
On 23/07/2018 19:29, Wang, Steven wrote:
> Dear all,
>
> Do you know any software available for aligning the center of geometry of each selected particle to the center of the particle-selection box? I need this centering for testing some algorithms, not for the regular cryo-EM map construction workflows. Thanks.
>
> Steven Wang
>
--
==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
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Emeritus Professor of Cryo-EM Data Processing
Leiden University
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Imperial College London
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