[3dem] negative staining artifact

[Linda Sandblad]

Hi all experts!!
I know, this do not look nice, but I would like to hear your opinion about this artifact. Tiny dark spots covering both sample and background, do you know what it is, were it comes from and how to avoid it? We have this bad background time to time from different labs…. It is a “normal” negative staining protocol: copper grids, formvar, carbon, glow discharge, UA.
I’m happy for your suggestions, Best Linda
[cid:0CCDA9FC-AEBF-4621-B208-6A9BFB2DF2B3 at lan]

Linda Sandblad
……………………………………………….
Department of Molecular Biology
Umeå University
+46 709324936

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