[3dem] 3dem Digest, Vol 122, Issue 69
Qiaohui Yang
qiaohui.yang at ymail.com
Mon Oct 16 19:23:50 PDT 2017
Dear all,
I have attached an EM image here for an example. Note the bubble like structures surrounding the cells. Thank you so much for your help!
Many thanks,Qiaohui
On Tuesday, 17 October 2017, 3:00, "3dem-request at ncmir.ucsd.edu" <3dem-request at ncmir.ucsd.edu> wrote:
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Today's Topics:
1. Gatan Multi-Specimen Single Tilt Cryo-Transfer Holder
(Roberto Marotta)
2. Re: Needing expert advice on cryosectioning for Tokuyasu
method) (Vlad Speransky)
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Message: 1
Date: Mon, 16 Oct 2017 14:05:34 +0000
From: Roberto Marotta <Roberto.Marotta at iit.it>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Gatan Multi-Specimen Single Tilt Cryo-Transfer Holder
Message-ID:
<394E95EBDD7E574B8D2123882082A58E6D13E229 at IITMXWGE015.iit.local>
Content-Type: text/plain; charset="us-ascii"
Hi,
I'm evaluating the Gatan 910 multi-specimens cryo-transfer holder we will eventually use in a Tecnai G2 F20 TWEEN microscope equipped with an automatic cryobox.
Any information you can provide me on this holder would be greatly appreciated.
Best,
Roberto Marotta
**************************************************************************
Roberto Marotta, PhD
Electron Microscopy Facility
Istituto Italiano di Tecnologia, IIT
Via Morego 30, 16163 Genoa, Italy
email: roberto.marotta at iit.it
t: +39 010 71781 897
f: +39 010 71781 236
P Please consider the environment before printing this e-mail.
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Message: 2
Date: Mon, 16 Oct 2017 07:57:58 -0700
From: "Vlad Speransky" <vlad_speransky at tedpella.com>
To: <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Needing expert advice on cryosectioning for
Tokuyasu method)
Message-ID: <02f201d3468f$284f0010$78ed0030$@tedpella.com>
Content-Type: text/plain; charset="utf-8"
Hi Qiaohui,
Is there an image you could share? Your description suggests the methylcellulose possibly getting old, but could be other things.
Best wishes,
Vlad
Vlad Speransky, PhD
Life Sciences Product Specialist
Ted Pella, Inc.
<http://www.tedpella.com/> http://www.tedpella.com/
530-243-2200 ext. 266
Cell 530-768-3953
<mailto:vlad_speransky at tedpella.com> vlad_speransky at tedpella.com
From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of qiaohui.yang at ymail.com
Sent: Sunday, October 15, 2017 2:28 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Needing expert advice on cryosectioning for Tokuyasu method)
Hi everyone,
I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!
Kind regards to all,
Qiaohui
Sent from Yahoo Mail on Android <https://overview.mail.yahoo.com/mobile/?.src=Android>
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