[3dem] Microtubule in vitro polymerisation
Prashant Singh
prash.byeo at gmail.com
Thu Nov 9 16:08:10 PST 2017
Hi Linda,
It might be worth trying tubulin from PurSolutions - it is cycled and cryopreserved.
You can find more info here: https://puresoluble.com/cycled-tubulin/ <https://puresoluble.com/cycled-tubulin/>
Thank you
Prash
*In the interest of full disclosure, I have a vested interest in PurSolutions.
> On Nov 9, 2017, at 3:33 PM, Linda Sandblad <linda.sandblad at umu.se> wrote:
>
> Hi!! I must mention, at the EM facility we have many beautiful samples, it’s just that I often think the artifacts are more interesting to discuss ;-)
>
> This is a question especially to all of you microtubule experts; When our students prepare mt from Cytoskeleton bovine tubulin it sometimes looks perfect, but sometimes it is all covered by those huge blobs, what do you think cause this artefact??
>
>
>
> Happy to hear your thoughts! Best Linda
>
> <170831_N5_MT01_0009_reduced.jpg>
>
>
> Linda Sandblad
>
> Department of Molecular Biology
> Umeå University
> Tel: +46 709324936
>
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