[3dem] contents of 3dem digest vol 123, issue 11: Microtubule in vitro polymerisation
Michelle Moritz
moritz at msg.ucsf.edu
Thu Nov 9 13:58:03 PST 2017
Hi Linda,
Regarding the blobs you sometimes get on your microtubules: It could be
aggregated tubulin that couldn't polymerize. Do you spin the tubulin at
high speed (100k x g, 10min in table-top ultracentrifuge) before
polymerizing? Also, we normally put the tubulin through at least one
additional polymerization/depolymerization cycle before freezing away in
small aliquots for use in experiments.
--
Michelle Moritz, PhD
Agard and Krogan Labs
UCSF MC2240, Rm S416
Dept. of Biochemistry & Biophysics
600 16th Street
San Francisco, CA 94158-2517
415-502-2930
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