[3dem] FEI-VPP phase change to opposite direction
jamon
jamonshi at gmail.com
Wed Jan 25 21:11:36 PST 2017
Dear Lister,
We just changed our FEI Volta Phase Plate on Krios. But after
activation, the phase changed to opposite direction, the FFT rings moves
outward instead of inwards as it should be. The gold particles all looks
all white as if in overfocus. I had never seen such behavior with
previous two VPPs we had. Did anybody have similar experience? Thanks in
advance.
--
Best Regards,
Jian SHI
Manager, Cryo Electron Microscopy Facility
Center for BioImaging Science
National University of Singapore
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