[3dem] VPP phase development curve
jamon
jamonshi at gmail.com
Thu Feb 23 18:26:31 PST 2017
Dear Lister,
Does anyone calibrated the VPP phase development curve during data
collection, using ctffind4.1.x or gCTF? I ran some test on Krios and
notice the phase changes rapidly from 0 degree to 90 degree with less
than <500e/A2, then reach 120 degree at 1000e/A2. Later the phase shift
moves much slower from 120 degree to 140 degree within 5000e/A2. It
suggest that the usable dose window is very narrow. Is there a way to
control phase shift stable at 90 degree instead of 120 degree? Your
suggestions are appreciated in advance.
Thanks and Regards,
Jian
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