[3dem] FFT question
Marin van Heel
marin.vanheel at googlemail.com
Fri Dec 29 12:27:12 PST 2017
Dear Gary
It could be high-frequency vibrations of your one holder but it can be a
number of other things as well. We have developed special software
within the IMAGIC-4D
system to diagnose such problems. If you want us to run a test on your
data we would need access to you entire 50 frames movie.
Cheers
Marin
==================================================================================
On 29/12/2017 17:23, Garry P Morgan wrote:
> Hi,
> we’re getting consistently clipped FFT’s on our TF30…the attached
> image is from a 50-frame, summed/aligned image of the carbon (of a
> cryo-grid). the drift numbers are reasonably low, there is no
> astigmatism. just wondering if any of you can provide a possible
> explanation of this? it definitely looks like drift or some sort of
> movement, right? we have 2 Gatan 626 holders, and it seems to only be
> happening with one of the holders…has anyone encountered a problem
> like this associated with a holder?
> thanks,
> Garry
>
> _______________________________________________
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> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
--
==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
tel: +55-19-3518-2316
mobile +55-19-981809332
(041-19-981809332 TIM)
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
marin.vanheel(A_T)lnnano.cnpem.br
and: mvh.office(A_T)gmail.com
--------------------------------------------------
Emeritus Professor of Cryo-EM Data Processing
Leiden University
Mobile NL: +31(0)652736618
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
email: m.vanheel(A_T)imperial.ac.uk
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