[3dem] Workshop announcement: Electron crystallography of 2D crystals of membrane proteins with 2DX, August 22-27, 2016
Henning Stahlberg
henning.stahlberg at unibas.ch
Wed May 11 04:30:24 PDT 2016
Hi,
Sorry, wrong dates: August 22-27, 2016. (Lectures August 23-26).
Henning.
Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62
> On 11 May 2016, at 10:49, Henning Stahlberg <henning.stahlberg at unibas.ch> wrote:
>
> Dear colleagues,
>
> We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.
>
> Topics include:
> Basics of 2D electron crystallography
> • Introduction to 2D electron crystallography
> • Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
> • Cryo-EM data collection of 2D crystals
> • Introduction to 2DX
> • Drift correction using ZORRO
> Basic image processing with 2DX
> • Determination of the defocus, crystal tilt geometry, crystal lattice
> • Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
> • 2D merging and 3D merging with reconstruction via weighted back projection
> • Automation of the processing pipeline
> Advanced topics in image processing with 2DX
> • Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
> • Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
> • Advanced usage of the 2DX backend library
> • Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
>
> Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at
> http://www.2dx.unibas.ch/workshop/2016
>
> For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
> The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve” basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,
>
> Nikhil Biyani,
> Ricardo Righetto,
> Robert McLeod,
> Henning Stahlberg.
>
> Henning Stahlberg, PhD
> Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
> Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
> http://c-cina.org | Tel. +41-61-387 32 62
>
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