[3dem] Floating carbon off of mica

Tue May 13 23:34:48 PDT 2014

Hey Isabel,
In order to let the carbon let go of the mica we breath on it (just like before you clean your glasses). The carbon lets go much easier. 

Best regards,
Linda

On 14-05-14, 3dem-request at ncmir.ucsd.edu wrote:
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> Today's Topics:
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>  1. Floating carbon off of mica? (Isabel Cylinder)
>  2. Re: Floating carbon off of mica? (Saikat Chowdhury)
>  3. Re: Floating carbon off of mica? (Smith, Phillip R.)
>  4. Professor of Structural Cryo-Electron Microscopy - position
>  (Matthew Weyland)
>  5. Re: Floating carbon off of mica? (Gonen PhD, Tamir)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 13 May 2014 21:11:04 +0000
> From: Isabel Cylinder <cylinder at ohsu.edu>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Floating carbon off of mica?
> Message-ID: <2CFF468925A8EC45A7DB6CA29A1E134D2EC343BE at EXMB08.ohsu.edu>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Hello-
> 
> I'm currently attempting to float a thin carbon film off of mica and onto EM grids. I was able to successfully complete the procedure before using a very thick layer generated by a very old carbon evaporator, but now we have a new machine and can key in layer thickness to the nanometer. That said, I'm creating a 10 nm layer of carbon on a 1"x3" piece of mica, and when I try to float the carbon into a Buchner funnel full of water, it crumbles and fragments and I am left with no useable pieces of carbon to float down onto my EM grids. My next step will be to try a 20 nm layer of carbon on a smaller piece of mica.
> 
> Does anybody have any experiences like this? How did you fix this problem? Any tips?
> 
> Any and all help is appreciated. Thank you!
> 
> Isabel Cylinder
> Research Assistant II
> Barklis Lab
> Molecular Microbiology & Immunology
> Oregon Health & Science University
> (503) 494-8098
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> ------------------------------
> 
> Message: 2
> Date: Tue, 13 May 2014 14:24:59 -0700
> From: Saikat Chowdhury <saikatbioinfo at gmail.com>
> To: Isabel Cylinder <cylinder at ohsu.edu>
> Cc: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Floating carbon off of mica?
> Message-ID:
> 	<CAAzOWR8D-UrxmQnpojYT3tPrkKFTcX9GysrTuX0b=Cts_shpdQ at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
> 
> Hi Isabel,
> Something that works for me is by letting the carbon coated mica sit in a
> 60C oven for a day or two. This helps in making the carbon film harder and
> more easier to handle. Also make sure that the freshly cleaved mica surface
> is very smooth and free of tiny mica fragments, dust or particulates.
> Good luck,
> Saikat
> 
> 
> On Tue, May 13, 2014 at 2:11 PM, Isabel Cylinder <cylinder at ohsu.edu> wrote:
> 
> > Hello-
> >
> > I'm currently attempting to float a thin carbon film off of mica and onto
> > EM grids. I was able to successfully complete the procedure before using a
> > very thick layer generated by a very old carbon evaporator, but now we have
> > a new machine and can key in layer thickness to the nanometer. That said,
> > I'm creating a 10 nm layer of carbon on a 1"x3" piece of mica, and when I
> > try to float the carbon into a Buchner funnel full of water, it crumbles
> > and fragments and I am left with no useable pieces of carbon to float down
> > onto my EM grids. My next step will be to try a 20 nm layer of carbon on a
> > smaller piece of mica.
> >
> > Does anybody have any experiences like this? How did you fix this problem?
> > Any tips?
> >
> > Any and all help is appreciated. Thank you!
> >
> > *Isabel Cylinder*
> > Research Assistant II
> > Barklis Lab
> > Molecular Microbiology & Immunology
> > Oregon Health & Science University
> > *(503) 494-8098 <%28503%29%20494-8098>*
> >
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> >
> >
> 
> 
> -- 
> SAIKAT CHOWDHURY
> (*Postdoctoral Research Associate --- Lander Lab*)
> Dept. of Integrative Structural and Computational Biology
> The Scripps Research Institute. HZ 102L
> 10550 N Torrey Pines Rd.
> La Jolla CA 92037
> 
> "If you think you know it, then you do not know it, and if you know that
> you cannot know it, then you know it"- G. N Ramachandran from Kenopanishad
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> ------------------------------
> 
> Message: 3
> Date: Tue, 13 May 2014 21:34:37 +0000
> From: "Smith, Phillip R." <smithp01 at nyumc.org>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Floating carbon off of mica?
> Message-ID: <541B1448-3E48-4281-9F11-5BA2BD2036A2 at nyumc.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Part of the problem might be poor control of the mica as you're floating the film off. 
> 
> This might help: http://www.laddresearch.com/grids-storage-boxes-support-film/smith-grid-coating-trough
> 
> -Ross Smith-
> 
> On May 13, 2014, at 5:24 PM, Saikat Chowdhury <saikatbioinfo at gmail.com> wrote:
> 
> > Hi Isabel,
> > Something that works for me is by letting the carbon coated mica sit in a 60C oven for a day or two. This helps in making the carbon film harder and more easier to handle. Also make sure that the freshly cleaved mica surface is very smooth and free of tiny mica fragments, dust or particulates.
> > Good luck,
> > Saikat
> > 
> > 
> > On Tue, May 13, 2014 at 2:11 PM, Isabel Cylinder <cylinder at ohsu.edu> wrote:
> > Hello- 
> > 
> > I'm currently attempting to float a thin carbon film off of mica and onto EM grids. I was able to successfully complete the procedure before using a very thick layer generated by a very old carbon evaporator, but now we have a new machine and can key in layer thickness to the nanometer. That said, I'm creating a 10 nm layer of carbon on a 1"x3" piece of mica, and when I try to float the carbon into a Buchner funnel full of water, it crumbles and fragments and I am left with no useable pieces of carbon to float down onto my EM grids. My next step will be to try a 20 nm layer of carbon on a smaller piece of mica. 
> > 
> > Does anybody have any experiences like this? How did you fix this problem? Any tips? 
> > 
> > Any and all help is appreciated. Thank you! 
> > 
> > Isabel Cylinder
> > Research Assistant II
> > Barklis Lab
> > Molecular Microbiology & Immunology 
> > Oregon Health & Science University 
> > (503) 494-8098
> > 
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> > 
> > 
> > 
> > 
> > -- 
> > SAIKAT CHOWDHURY
> > (Postdoctoral Research Associate --- Lander Lab)
> > Dept. of Integrative Structural and Computational Biology
> > The Scripps Research Institute. HZ 102L
> > 10550 N Torrey Pines Rd.
> > La Jolla CA 92037
> > 
> > "If you think you know it, then you do not know it, and if you know that you cannot know it, then you know it"- G. N Ramachandran from Kenopanishad
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> 
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> ------------------------------
> 
> Message: 4
> Date: Wed, 14 May 2014 09:11:52 +1000
> From: Matthew Weyland <matthew.weyland at monash.edu>
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Professor of Structural Cryo-Electron Microscopy -
> 	position
> Message-ID: <5372A6B8.3040101 at monash.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> 
> Email: matthew.weyland at monash.edu
> Name: Matthew Weyland
> 
> Organization: Monash University
> 
> Professor of Structural Cryo-Electron Microscopy - position available
> 
> The Monash-Warwick Professor of Structural Cryo-Electron Microscopy
> 
> A joint position with
> Monash University, Australia (80%)and Warwick University, UK (20%)
> 
> http://jobs.monash.edu.au/jobDetails.asp?sJobIDs=523391
> 
> Deadline has some flexibility.
> 
> For enquiries, please contact:
> 
> Professor James Whisstock
> ARC Federation Fellow
> Department of Biochemistry
> Monash University
> Clayton, Melbourne, VIC, 3800
> Australia
> Email: james.whisstock at monash.edu
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Wed, 14 May 2014 00:12:19 +0000
> From: "Gonen PhD, Tamir" <gonent at janelia.hhmi.org>
> To: Isabel Cylinder <cylinder at ohsu.edu>
> Cc: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Floating carbon off of mica?
> Message-ID:
> 	<E839F35231EF25449A870946FA3990220102DC8569 at JFMB1.hhmi.org>
> Content-Type: text/plain; charset="us-ascii"
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