[3dem] Movie-mode data collection and Intra-movie alignments in cryo-EM
Marin van Heel
m.vanheel at imperial.ac.uk
Tue Mar 25 06:19:26 PDT 2014
Dear All,
With the advent of the ever more popular "movie mode" data collection
and "intra movie" alignments in single-particle cryo-EM, I would like to
remind our community of this important 30-year old paper by our late
friend Wolfgang Kunath:
*TIME-RESOLVED LOW-DOSE MICROSCOPY OF GLUTAMINE SYNTHETASE MOLECULES*
**
*Ultramicroscopy 13 (1984) 241-252*
W. KUNATH, K. WEISS, H. SACK-KONGEHL, M. KESSEL and E. ZEITLER
*"Time sequences of low-exposure images from the enzyme molecules of
glutamine synthetase have been recorded digitally using an on-line
recording system. Processing the images results in a time sequence of
averaged molecule images which, in the very beginning, exhibit the
molecule structure still undamaged by electron irradiation. Different
sources of the noise which obscures structural details, such as shot
noise, stain embedding and radiation damage, are investigated."*
All the best,
Marin
--
================================================================
Prof. Dr. Ir. Marin van Heel
Professor of Cryo-EM Data Processing
Leiden University
NeCEN Building Room 05.27
Einsteinweg 55
2333 CC Leiden
The Netherlands
Tel. NL: +31(0)715271424 // Mobile NL: +31(0)652736618
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
and: mvh.office(A_T)gmail.com (Élen Tomazela)
----------------------------------------------
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
Biochemistry Building (Room 512)
South Kensington Campus
London SW7 2AZ, UK
email: m.vanheel(A_T)ic.ac.uk
Tel. UK: +44(0)2075945316 //Mobile: +44(0)7941540625
----------------------------------------------
Visiting Professor at:
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/ABTLuS, Campinas, Brazil
mobile phone +55-19-99369051
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