[3dem] 3dem Digest, Vol 79, Issue 11
Tapu Shaikh
shaikh at ceitec.muni.cz
Wed Mar 12 14:29:45 PDT 2014
On 03/12/2014 10:07 PM, Sindelar, Charles wrote:
> Hi Qiu-Xing, the experiment that Ed refers to is one of the better ones you can do- the FSC between the PDB-generated map, and your experimental map. Particularly if you do only rigid-body fitting (not flexible fitting), the 0.5 cut-off will give you a conservative estimate of the resolution- i.e. it is difficult or impossible to over-estimate the resolution in this way.
I wrote to Qui-Xing off-list because I didn't know the math in detail,
but Pawel had given a talk where he said that, when computing the
cross-resolution between an atomic model and an EM map, a SNR of 1
corresponds to a FSC of 1/sqrt(2) rather than 1/2. [Specifically, the
math I didn't understand was the origin of the exponent 2 in
SNR=FSC^2/(1-FSC^2).] If that's the case, a 0.5 cutoff may not be such
a conservative estimate.
-Tapu
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