[3dem] amplitude contrast valuesHi,
Sacha De Carlo
sachadecarlo at yahoo.com
Mon Jul 21 13:18:20 PDT 2014
Hi David,
0.2 - 0.3 is not unreasonable for (cryo) neg.st. I had the values written in one of my reviews, but I don’t remember which one :)
Cheers,
Sacha
On Jul 21, 2014, at 7:02 PM, David Gene Morgan <dagmorga at indiana.edu> wrote:
> Hi,
>
> I'm trying to do some automated defocus determination (initially trying Niko's ctffind3 program) and would like some input on appropriate amplitude contrast values for various types of samples. I know that ice embedded images usually are said to have an amplitude contrast of 0.07 to 0.1. I also know that with protein/nucleic acid complexes in ice, amplitude contrast may need to be set a bit higher (say to as much as 0.15 for some data I worked on awhile ago).
>
> But I don't have any sort of feeling for appropriate values of amp;itude contrast for different sorts of negative stains (either cryo negative stain or standard negative stain). It should be "high," but I don't know how high might be appropriate.
>
> If anyone has either some practical or theoretical insight into this, I'd appreciate some suggestions. Also, does anyone know about an appropriate amplitude contrast for a standard Au/Pd waffle grid?
>
> Thanks in advance for all your help.
>
> --
> David Gene Morgan
> Electron Microscopy Center
> 047D Simon Hall
> IU Bloomington
> 812 856 1457 (office)
> 812 856 3221 (3200)
> http://bio.indiana.edu/~cryo
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