[3dem] Calcium free filter paper
rkhayat at ccny.cuny.edu
rkhayat at ccny.cuny.edu
Fri Aug 29 17:49:00 PDT 2014
Hi,
How about adding EDTA to your complex to chelate the calcium,
or wash the filter paper with EDTA and let it dry in a vacuum
before use.
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
www.khayatlab.org
---- Original message ----
>Date: Fri, 29 Aug 2014 18:22:06 -0400
>From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Jing Wang
<jing at bu.edu>)
>Subject: [3dem] Calcium free filter paper
>To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
>
> Hi there,
> I'm working with a complex of calcium sensor
> protein, which dissembles upon exposure to calcium.
> The complex fell apart after freezing and I
> suspected it might due to the** residual calcium
> from the vitrobot blotting paper. Is there a
> commercially available calcium-free filter paper or
> is there a protocol for cleaning up the regular
> filter paper? Any comment will be greatly
> appreciated!!
> Jing
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