[3dem] "leopard skin" ice
Lynne.Waddington at csiro.au
Lynne.Waddington at csiro.au
Sat Nov 23 16:04:05 PST 2013
________________________________
Hi All
This has been very interesting. In Australia it is definitely snake-skin, no shaving necessary... I have interpreted it in the same way as Willem, and I do see it increasing over time, especially toward the end of the day after I’ve loaded a number of cryo grids. The cure has been to do a really good cryo cycle overnight and in the morning everything is fine again.
However, on Friday my first grid of the day was extremely snakey, but subsequent grids were fine, so something else must be going on.
I am following this post with great interest!
Lynne
Lynne Waddington
CryoTEM facility
CSIRO Materials Science and Engineering
343 Royal Parade
Parkville
Victoria 3052
AUSTRALIA
Phone: +61 3 9662 7205
Fax: +61 3 9662 7101
e-mail: lynne.waddington at csiro.au<UrlBlockedError.aspx>
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Willem Tichelaar
Sent: Friday, 22 November 2013 9:31 AM
To: 3dem at ncmir.ucsd.edu
Subject: PS Re: [3dem] "leopard skin" ice
PS See also Dubochet et al. (1988) Quart. Rev. Biophys. 21, pp. 129-228, Fig. 32 c and d
Hi Frank,
I thought that the contamination with a leopard skin pattern arises in the microscope when the vacuum is not clean i.e. when the partial water pressure near the specimen is not low enough. Then, the contamination that you see should increase with time though.
Good luck,
Willem.
On 11/20/13 6:23 PM, frankpolzer at physik.hu-berlin.de<mailto:frankpolzer at physik.hu-berlin.de> wrote:
Hi again,
Please find attached an example image of the ice I observed.
Cheers,
Frank
Dear all,
During the last weeks, I frequently observe what I believe is so-called
leopard skin ice in my vitrified samples (plunge frozen in ethane using
Mark IV Vitrobot).
What again is the reason for this type of ice and how can this be avoided?
Thanks for your help in advance,
Frank
Dr. Frank Polzer
TEM Group
Insitute of Physics
Humboldt Universität zu Berlin
Newtonstraße 15
12489 - Berlin
Tel.: +49 30 2093-4995 (office)
Tel.: +49 30 2093-7829 (TEM)
Fax: +49 30 2093-7886
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Dr. Willem Tichelaar
Richard-Drach-Str. 5
69123 Heidelberg, Deutschland
phone: +49 (0)6221 779409; fax: +49 (0)6221 779411
e-mail: w.tichelaar at online.de<mailto:w.tichelaar at online.de>
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