[3dem] How to determine a symmetry of a ring particle?
Sharon G. Wolf
Sharon.Wolf at weizmann.ac.il
Tue Jan 15 00:40:59 PST 2013
I used Rotastat in the past. It's very important to collect "background" data in the same quantity and imaging conditions that the images of the particles are taken.
Best,
Sharon
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Bernard Heymann
Sent: Monday, 14 January, 2013 17:47
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] How to determine a symmetry of a ring particle?
Dear Alexander
Another alternative is the method used in:
Ultramicroscopy. 1995 Sep;60(2):219-28
It can handle polymorphism.
You can find the application here:
http://lsbr.niams.nih.gov/lsbr/RotaStat.html
Best
Bernard
--------------------------------------------------------------------------------------------------------------------
J. Bernard Heymann Ph.D.
LSBR/NIAMS/NIH
Rm 1515, 50 South Dr, NIH
Bethesda MD 20892-8025
Tel. 301-451-8241
On Jan 14, 2013, at 4:30 AM, Philip Köck <Philip.Koeck at ki.se<mailto:Philip.Koeck at ki.se>> wrote:
That's another good idea.
You might actually have eigen images already. They could be one of the outputs of the 2D classification
you did in Eman.
Look at Dube et al. to learn how to interpret the eigen images, if you find them.
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of michael
Sent: 14 January 2013 10:23
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] How to determine a symmetry of a ring particle?
Dear Alexander,
Dube et al. 1993 are using multivariate statistical analysis (MSA) to estimate the symmetry properties by looking at the eigenimages.
A description of the method can be found here: www.singleparticles.org/methodology<http://www.singleparticles.org/methodology> . You can download the PDF file "Multivariate Statistical Analysis in Single Particle (Cryo) Electron Microscopy". The related chapter is "12. MSA symmetry analysis".
The original paper is: Dube P, Tavares P, Lurz R and van Heel M EMBO J. (1993) 1303-1309.
I also want to refer to Pawel's hint: the method is a symmetry estimation and not a determination.
Regards
Michael
--
======================
Michael Schatz
Image Science Software GmbH
Gillweg 3
D - 14193 Berlin
Germany
Besuchereingang / Entrance:
Hubertusallee
Tel: +49 30 8909 5326
Fax: +49 30 8909 5321
URL: www. ImageScience .de
Geschäftsführer/Managing Director
Michael Schatz
HRB: 33106 Berlin-Charlottenburg
VAT/USt-Id-Nr. DE 136613484
======================
_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20130115/f5c2e3bd/attachment.html
More information about the 3dem
mailing list