[3dem] freezing tube using Leica HPM100
Chen Chen
c.chen0306 at gmail.com
Wed Feb 13 21:45:51 PST 2013
Sounds great! I'll try it out.
Thanks,
Chen
On Feb 14, 2013, at 1:41 PM, Cedric Bouchet-Marquis wrote:
> Yes it should be enough. When in the microtome, you could trim for ~100 micron this side of the tube and assess if you pressed it enough or not
>
> Cedric
> Sent from my iPhone
>
> On Feb 13, 2013, at 9:34 PM, "Chen Chen" <c.chen0306 at gmail.com> wrote:
>
>> May I know how you seal the end? Normally I just clamp the tube to make the end flat. Do you think that's enough?
>>
>> Thanks,
>> Chen
>> On Feb 14, 2013, at 1:18 PM, Cedric Bouchet-Marquis wrote:
>>
>>> Yes, you have to seal the side that faces the machine when mouting it in its "platic" holder.
>>>
>>> Best
>>>
>>> Cedric
>>>
>>> Sent from my iPhone
>>>
>>> On Feb 13, 2013, at 9:16 PM, "Chen Chen" <c.chen0306 at gmail.com> wrote:
>>>
>>>> Dear Lists,
>>>>
>>>> Has anyone tried to high pressure freeze samples in a tube using Leica HPM100? I am wondering if I need to seal one or both ends of the tube before freezing. Thanks in advance.
>>>>
>>>> Cheers,
>>>> Chen_______________________________________________
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