[3dem] Gatan cryo-holder question
Ruben Diaz
pindusito at gmail.com
Sun Dec 15 18:07:46 PST 2013
Hi David,
I really have no idea why this happens (probably Maxwell's demon doing one of its tricks), but I also have seen this problem with the tomography cryoholder. The way I got rid of this problem is by putting a tiny dab of formvar in chloroform (a 1% solution works fine) on the head of the screw. The tiny bit that stays at the tip of a toothpick is enough, as it runs into the screw and most of its surfaces, fixing them more or less securely.
Happy holidays to you too!
Ruben.
*******************************
Ruben Diaz-Avalos, Ph.D.
Department of Molecular and Cellular Biology,
Briggs Hall,
University of California, Davis,
1 Shields Avenue,
Davis, CA 95616
Phone: (530)752-8282
Cell: (917)515-6353
*******************************
On Dec 15, 2013, at 11:37 AM, David Gene Morgan <dagmorga at indiana.edu> wrote:
> Hi,
>
> In the last six months, we have lost a screw from the tip of both our
> Gatan 626 and Gatn 914 cryo-holders. Has anyone else had similar
> problems, and does anyone have suggestions for why this may be
> happening? Thanks, and Happy Holidays!
>
> --
> David Gene Morgan
> Electron Microscopy Center
> 320C Simon Hall
> IU Bloomington
> 812 856 1457 (office)
> 812 856 3221 (3200)
> http://bio.indiana.edu/~cryo
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
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