[3dem] IHRSR in SPARX
Penczek, Pawel A
Pawel.A.Penczek at uth.tmc.edu
Wed Aug 28 13:20:18 PDT 2013
For those who use or plan to use IHRSR (real-space helical reconstruction) implementation in SPARX (sxihrsr) an important note.
We introduced sxhelixboxer (a modified version of e2boxer) that is closely integrated with sxihrsr.
In particular, it has built-in CTF estimation and it also stores in segments's headers information
required for validation of IHRSR results.
The documentation can be found here:
http://sparx-em.org/sparxwiki/sxhelixboxer
and an example how to carry out helical reconstruction is here:
http://sparx-em.org/sparxwiki/Runthrough_helical_example
Description of the methodology:
Behrmann, E., Tao, G., Stokes, D.L., Egelman, E.H., Raunser, S., and Penczek, P.A., 2012. Real-space processing of helical filaments in SPARX. J Struct Biol 177, 302-313.
Regards,
—
Pawel A. Penczek, Ph.D.
Professor
Structural Biology Imaging Center, Director
The University of Texas
phone: 713-500-5416
fax: 713-500-0652
http://www.uth.tmc.edu/bmb/faculty/pawel-penczek.html
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