[3dem] prepare Formvar Grids
Denis Chaix
denis.chaix at ibs.fr
Thu Sep 27 13:30:40 PDT 2012
Hello
We have actualy some troubles to prepare Formvar films.
In fact it's impossible to release the film from the glass slide.
Here you can find our protocol
Formvar solution. 0,4% in chloroform .
Pour the solution into a clean Coplin jar
Dip pre-cleaned slides ( rub thoroughly with Kimwipes) into beaker.
Drain corner on side of beaker.
Blot edge of slide on filter paper.
Score the edges of the Formvar film with an acetone-cleaned razor blade.
Breathe over the length of the slide to loosen the film.
Float the film off onto a clean water surface by slowly immersing the
slide into the water at a 45° angle.
Have you some tricks or tips to improve this protocol and help the
release of the film ?
For example, have you some ideas like special brand of slides, or a
way to clean the slides.
Thanks
Denis
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Chaix Denis <denis.chaix at ibs.fr>
Microscopie Electronique et Méthodes
<http://www.ibs.fr/groupes/groupe-microscopie-electronique-et/>
Room 6234
Institut de Biologie Structurale UMR-5075 <http://www.ibs.fr/spip.php>
41 Rue J. Horowitz
38027 Grenoble Cedex 1,France
Tel: +33.4.38.78.01.59.
Fax: +33.4.38.78.54.94.
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