[3dem] Image file format of Eagle 4K CCD and conversion
Sacha De Carlo
sachadecarlo at yahoo.com
Thu Aug 23 08:24:11 PDT 2012
You can export as TIFF (Full Resolution) from TIA directly, or you can import the *.EMI file in Digital Micrograph and use the Integer 2 byte unsigned data type and set an offset of 126 in the binary offset (assuming a 4096 x 4096 size and depth of 1).
I believe that the free EM2EM image converter from IMAGIC allows you to convert directly as well (I will ask Marin if it works).
Best regards,
Sacha
=============================
Dr. Sacha De Carlo
Sr. Res. Scientist
FEI Company
________________________________
From: "hohu78 at gmail.com" <hohu78 at gmail.com>
To: 3dem at ncmir.ucsd.edu
Sent: Thursday, August 23, 2012 4:28 PM
Subject: [3dem] Image file format of Eagle 4K CCD and conversion
Dear all,
I have installed the Eagle 4K CCD camera from FEI on Tecnai G2
spirit.
Because I only have experienced Gatan camera, I’m struggling to figure out
this camera and software.
Questions are
1. How to convert emi file format which is the basic option in eagle camera
to MRC or IMAGIC format?
Is there any available free software?
2. Using export option in software, I can also save image as several
formats,
like Tif (full resolution), Bmp(full resolution), JPG
(full resolution), Raw binary (.bin), True image (.ebn).
Which file format is the best one for single particle
analysis without losing the information of image?
Anyone who have experiences, give me valuable suggestions, please.
Many thanks,
Best regards,
Homin Kim
Homin Kim,
Ph D.
Korea Advanced Institute of Science and Technology (KAIST)
Graduate
School of Medical Science and Engineering
BioMedical Research Center(E7)
Room 2102, KAIST
291 Daehak-ro, Yuseong-gu
Daejeon, 305-701, Republic of
Korea
Phone: +82-10-7184-5521(Cellphone), +82-42-350-4244 (Office)
FAX :
+82-42-350-4240
Email: hm_kim at kaist.ac.kr
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