[3dem] Is there an asymmetric biological sample out there that
reveals handedness at CET resolution?
Christian Geiss
geiss at biophysik.org
Tue Aug 7 11:27:44 PDT 2012
Hello,
I am a Phd student, do cryo electron tomography and have a problem
determining the handedness of my sample, because viewers for tomograms
like Amira, Matlab-based scripts can invert the handedness depending if
you look at your tomogram from top to bottom or vice versa. Since those
programs behave to some extent like black boxes, the easiest would be to
have a nice asymmetric biological sample where you know the handedness
and that the latter can be judged quite easily from a reconstruction,
meaning without further processing like subtomogram averaging.
So far, I can only think of bacterial ribosomes since they are
asymmetric and quite big, but for cryoET the resolution would be not
reasonable to resolve the handedness at all. Thus I could need in the
ideal case e.g. something like a large complex that links each other to
repetitive units with a known handedness.
I know its a tough question, but maybe someone can give creative input
here? I would highly appreciate it!
Best regards,
C. Geiss
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