[3dem] DMSO and vitrified ice digest
rkhayat
rkhayat at scripps.edu
Thu Jul 14 07:49:52 PDT 2011
Hi Everyone,
I received the following replies regarding my question:
What is the maximum amount of DMSO that can be present in a sample
when making vitrified grids for cryo-EM?
1. Ron Milligan: None, try to avoid any DMSO as it quickly bubbles.
2. Jeol Meyerson: 1% DMSO used in Chipman et al. PNAS 1996, vol. 93
7502-7506.
3. I found: 2% DMSO used in Zhang et al. PNAS 2009, vol 106 4653-4658
I'm having issues getting good ice, so I don't have any experience
with DMSO yet.
Regards,
Reza
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