[3dem] FIB-SEM
Emily Knauth
e.knauth at imb.uq.edu.au
Mon Jan 24 12:46:40 PST 2011
Hi Abrahan,
How are you samples fixed?
Cheers,
Emily Knauth
Institute for Molecular Bioscience
Division of Chemistry and Structural Biology
The University of Queensland
306 Carmody Road
St Lucia 4072
Brisbane, QLD
Australia
Phone: + 61 7 3346 2325
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Abrahan Hernandez
Sent: Tuesday, 25 January 2011 2:38 AM
To: 3dem at ucsd.edu
Subject: [3dem] FIB-SEM
Dear 3dem community,
I am performing data acquisition with a FIB-SEM (Focus ion beam- Scanning electron microscope) of metaphase chromosomes in a whole cell. My samples are stained pre-embedding with tannic acid (0.15%, for 1 min), Osmium tetra-oxide (2%, for 1 hour) and uranyl acetate (1%, fir 1 hour). We can see our structures (metaphases chromosomes) but the contrast is not so good, therefore I would like to ask you if you have any recommendation for improve the pre-embedding staining.
Best
-----
Abrahan Hernandez Hernandez, PhD
Postdoc
Department of Cell and Molecular Biology (CMB)
Berzelius Väg 35
Karolinska Institutet
S-171 77 Stockholm, Sweden
Phone: +46 (0)8 5248 7396
Email: abrahan.hernandez at ki.se
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