[3dem] Vitrobot mark III vs mark IV
Bob Grassucci
rg2502 at columbia.edu
Fri Apr 8 09:36:18 PDT 2011
Hi Martin,
Unfortunately it depends on how the zero force was set on the Mark IV
and there is no standard way they are calibrated. While the mark 3
changed the position of the grid relative to the V of the blot pads the
Mark 4 changes the distance the pads are away from each other. In other
words if the the zero force on one mark 4 is set closer than another
it's blot force for a particular setting will be stronger than for
another where zero was a bit further apart. Initially the Mark 4's zero
force was not set at all which lead to irreproachable results.
I can not recall now is 3 mm deeper into the v than -3mm ? If so I
would start with a positive blot force and once you get the right force
do not change it with similar grids and sample (this can also change
with sample and grid type). I hope this helps. Good luck.
Regards
Bob
On 4/8/2011 11:53 AM, MP Cheung wrote:
> Hi All,
>
> Does anyone know how the 'Blotforce' setting on the Mark IV compares to
> the 'Offset' setting on the Mark III? If I have previously used a 3mm
> offset in the Mark III, what blotforce would I need to set in the Mark IV
> to replicate the same conditions?
>
> Thanks,
>
> Martin
>
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>
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