[3dem] Registration and 3-D Reconstruction
Steven Ludtke
sludtke at bcm.edu
Tue Jul 7 08:11:40 PDT 2009
It's a very active field of research. The question is which
methodology you are interested in, each has its own specialties:
- single particle reconstruction - sets of very noisy images of
individual ~identical nanoscale objects -> 3D reconstruction
- 2D crystallography - 3D reconstructions from crystals, like x-ray
crystallography, but with 2-D rather than 3-D crystals
- helical reconstruction - reconstruction using packed arrays with
helical symmetry
- tomography - VERY noisy 3D reconstructions of possibly unique
objects, if the objects are ~identical then 3D registration and
averaging can proceed
I would suggest starting out with Joachim Frank's introductory book :
"Three-dimensional Electron Microscopy", then perhaps looking at the
special issue of
JSB from a couple of years ago. The one containing this:
Tang, G., Peng, L., Baldwin, P.R., Mann, D.S., Jiang, W., Rees, I. &
Ludtke, S.J., 2007, EMAN2: an extensible image processing suite for
electron microscopy, Journal of structural biology, 157(1), pp. 38-46.
It has articles on many of the current software packages. My group
develops one of them (EMAN). There is some raw data available from our
website
http://ncmi.bcm.edu
----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor
Co-Director National Center For Macromolecular Imaging
Dept of Biochemistry and Mol. Biol.
Baylor College of Medicine
sludtke at bcm.edu
stevel at alumni.caltech.edu
http://ncmi.bcm.edu/~stevel
On Jul 7, 2009, at 4:53 AM, Arthur Goshtasby wrote:
>
> Hello everyone,
>
>
> I am a new member and my interest is in image registration and 3-D
> reconstruction. My background is actually computer vision but have
> recently become interested in microscopic image analysis. I have
> found such image fascinating. I am wondering if anyone else is
> interested in or currently working on the problem. I have some
> methodologies worked out for registration and 3-D reconstruction and
> would like to try them on different data sets. If you are working
> with microscopic image sets and don't mind sharing a set or two with
> me, I promise to share the obtained results and the methodology that
> is employed to obtain them with you? I can be reached via phone
> (937-775-5170) or e-mail (arthur.goshtasby at wright.edu).
>
>
> Thank you,
> Arthur Goshtasby _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
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