[3dem] Contamination problem on cryo grids
Bill Tivol
tivol at caltech.edu
Fri Sep 19 09:41:33 PDT 2008
On Sep 19, 2008, at 4:41 AM, Anselm Kusser wrote:
>
>> We are repeatedly experiencing problems with significant amount of
>> contamination on our grids (follow the links to have a look at some
>> example images).
> What you are seeing is not contamination, it is what we term
> "splotchy ice" which occurs if there are contaminants on the carbon
> causing the carbon to be hydrophobis not hydrophyllic.
Dear Anselm,
Another possibility is that there is a contaminant in the ethane. We
sometimes see this when the ethane tank is nearly empty.
Yours,
Bill Tivol, PhD
EM Scientist
Ultrafast EM Facility
Noyes Laboratory, MC 127-72
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu
More information about the 3dem
mailing list