[3dem] Tecnai script
William Rice
rice at nysbc.org
Thu May 22 06:16:45 PDT 2008
Hi,
Either SerialEM (http://bio3d.colorado.edu/SerialEM/) or Leginon
(http://ami.scripps.edu/software/leginon/) will do what you want. We are
using both on our Tecnai F20. Both are very well supported.
Bill
Eduardo Sanz-Garcia wrote:
> I was thinking on writing a script for a Tecnai F30 that would read a
> text file that store the good spots inside a grid square, move to them,
> and take a defocus pair.
> Before writing it, I thought it would be best to ask if someone has
> already written something similar, and if this approach sounds reasonable.
> I use lacey carbon, therefore, spotscan, I believe, doesn't fit my needs.
> I also found that the focus level doesn't vary so much inside each grid
> square so I do not need to refocus at every step.
>
> The idea is the following, for those familiar with the Technai F30:
>
> 1. Use Stage2 panel to store the good spots inside a grid square (in
> Search mode)
> 2. Save the spots in a text file.
> 3. Start the script to read the locations.
> 4. Move to the first.
> 5. Go to Exposure mode.
> 6. Take a pair of pictures (different defocus).
> 7. Move to the next spot.
>
> Thank you very much
>
>
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