[3dem] Single-sided blotting in Vitrobot?
Fred Sigworth
fred.sigworth at yale.edu
Wed Dec 10 13:41:19 PST 2008
Has anyone had success in using the Vitrobot to freeze a specimen that
needs blotting only on one side? On the one hand we have specimens
with ultra-thin carbon over a holey film. The vitrobot quite reliably
breaks the films (perhaps due to dry filter-paper fibers contacting
them?). On the other hand, we have streptavidin crystals spanning
holes, where the crystals are formed on a lipid monolayer which
presents a dry, hydrophobic surface on one side. It would be great if
we could use the Vitrobot to freeze these things.
-Fred Sigworth
More information about the 3dem
mailing list